PFA fixed hemi-brains were cryopresevered and sectioned coronally at 40 uM, and stored as 1 in 12 slices throughout the brain. Immunostains were performed for IBA1/Thio-S, GFAP/S100/Thio-S, and NeuN/Ctip2/Thio-S to visualise microglia, astrocytes, and neurons respectively at every 12th section throughout the brain. Slices were then imaged using a Zeiss Slidescanner, and then exported and feature counts quantified using Bitplane Imaris. Detailed methodology is available here (insert link). Aggregate data is shown below, and all images can be viewed and explored by clicking on the relevant links below.